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After the patient is pronounced dead by a physician, the body is wrapped in a sheet or shroud and transported to the morgue, where it is held in a refrigeration unit until the autopsy. Autopsies are rarely performed at night, but they are typically performed between 8 am and 4 pm every day, including weekends and holidays. In medium-size and large hospitals, the autopsy is done on the premises in a autopsy suite, which is either within or adjacent to the morgue. Small hospitals that do not have autopsy suites may arrange for autopsies to be done at a larger hospital. Yet other hospitals out in the country can only offer autopsies by having them done at funeral homes. Doing an autopsy at a funeral home is one of the most dreaded things a pathologist has to face.
There has been a general belief that some dieners also take payment under the table for notifying funeral homes of deaths in the hospital (so that the funeral home can send an agent out to approach the family), but I am not aware of any cases where this allegation was proved. From my own experiences, I know that in some cities the funeral home business is extraordinarily competitive, and I am aware of one case where agents of two funeral homes got into a physical altercation in the morgue over the disposition of a body that each claimed.
The other individual directly involved in the autopsy is the PROSECTOR. This is the individual who is in charge of the actual dissection. In small hospitals, the prosector is a Board-certified pathologist, an MD or DO (osteopath) who has undergone a four- or five-year residency in the specialty of pathology, specifically anatomic pathology. In university- based hospitals with teaching programs, the prosector is a pathology resident (a physician who is training to be a pathologist) or a medical student taking an elective rotation in pathology. In larger non-university-based hospitals covered by large pathology groups, the prosector may be a pathologist's assistant. The "PA" is typically a graduate of an associate or baccalaureate program which provides training in several areas of pathology, especially those that involve "hands-on" activities, such as autopsy dissections, dissections of specimens removed at surgery, specimen photography, and video applications. PA's enjoy excellent pay and benefits (US$40,000 to start) in their little-known area, and the demand for PA's continues to exceed supply.
Other individuals may be present at the autopsy, usually for educational opportunities. These may include the attending or consulting physicians, residents, medical students, nurses, respiratory therapists, and others involved in direct patient care.
The prosector and diener wear fairly simple protective equipment, including scrub suits, gowns, gloves (typically two pair), shoe covers, and clear plastic face shields. Some facilities have sealed-environment "space suits," but I think one is more likely to infect himself as a result of the clumsiness lent by these suits than if he were dressed more lightly in the interest of nimbleness.
The body is then measured. Large facilities may have total- body scales, so that a weight can be obtained. The autopsy table is a waist-high aluminum fixture that is plumbed for running water and has several faucets and spigots to facilitate washing away all the blood that is released during the procedure. Older hospitals may still have porcelain or even marble tables. The autopsy table is basically a slanted tray (for drainage) with raised edges (to keep blood and fluids from flowing onto the floor). After the body is positioned, the diener places a "body block" under the patient's back. This rubber or plastic brick-like appliance causes the chest to protrude outward and the arms and neck to fall back, thus allowing the maximum exposure of the trunk for the incisions. The prosector checks to make sure that the body is that of the patient named on the permit by checking the toe tag or patient wristband ID. Abnormalities of the external body surfaces are then noted and described, either by talking into a voice recorder or making notes on a diagram and/or checklist.
With the Y incision made, the next task is to peel the skin, muscle, and soft tissues off the chest wall. This is done with a scalpel. When complete, the chest flap is pulled upward over the patient's face, and the front of the rib cage and the strap muscles of the front of the neck lie exposed. Human muscle smells not unlike raw lamb meat in my opinion. At this point of the autopsy, the smells are otherwise very faint.
An electric saw or bone cutter (which looks a lot like curved pruning shears) is used to open the rib cage. One cut is made up each side of the front of the rib cage, so that the chest plate, consisting of the sternum and the ribs which connect to it, are no longer attached to the rest of the skeleton. The chest plate is pulled back and peeled off with a little help of the scalpel, which is used to dissect the adherent soft tissues stuck to the back of the chest plate. After the chest plate has been removed, the organs of the chest (heart and lungs) are exposed (the heart is actually covered by the pericardial sac).
Before disturbing the organs further, the prosector cuts open the pericardial sac, then the pulmonary artery where it exits the heart. He sticks his finger into the hole in the pulmonary artery and feels around for any thromboembolus (a blood clot which has dislodged from a vein elsewhere in the body, traveled through the heart to the pulmonary artery, lodged there, and caused sudden death. This is a common cause of death in hospitalized patients).
The abdomen is further opened by dissecting the abdominal muscle away from the bottom of the rib cage and diaphragm. The flaps of abdominal wall fall off to either side, and the abdominal organs are now exposed.
A cut is them made above the larynx, detaching the larynx and esophagus from the pharynx. The larynx and trachea are then pulled downward, and the scalpel is used to free up the remainder of the chest organs from their attachment at the spine. The diaphragm is cut away from the body wall, and the abdominal organs are pulled out and down. Finally, all of the organs are attached to the body only by the pelvic ligaments, bladder, and rectum. A single slash with the scalpel divides this connection, and all of the organs are now free in one block. The diener hands this organ bloc to the prosector. The prosector takes the organ bloc to a dissecting table (which is often mounted over the patient's legs) and dissects it. Meanwhile, the diener proceeds to remove the brain.
The diener takes an electric saw (typically called a "Stryker saw," even if it's not manufactured by Stryker) and makes cuts around the equator of the cranium. This cut must be deep enough to cut all the way through the skull, but not so deep that the brain is cut (this takes some skill). Typically, the cut is not totally straight but has a notch so that the skull top (calvarium) will not slide off the bottom half of the skull after everything is sewn back up. After this cut, the calvarium is removed and set aside. As the calvarium is lifted off, there is a very characteristic sound that is sort of a combination of a sucking sound and the sound of rubbing two halves of a coconut together. The best recorded representation of this sound that I have heard is in the brain transplant scene of the film _Robocop II_.
The outer layer of the meninges (the coverings of the brain), called the dura, stays with the calvarium, so that the top of the brain is now fully exposed. After the chore of getting to it, it is a relatively easy matter to get the brain out. There are no tough ligaments that hold the brain in, so really all that needs to be done is to cut the spinal cord and the dural reflections that go between the cerebellum and cerebrum (called the tentorium). The brain is then easily lifted out.
Since the brain is very soft and easily deformable, it is not manipulated at the time of the autopsy. Instead it is hung up by string in a large jar of formalin (a 10% solution of formaldehyde gas in buffered water) for two weeks or longer. The action of formaldehyde is to "fix" the tissue, not only preserving it from decay, but also causing it to become much firmer and easier to handle without deforming it. The reason that it is suspended by string is to prevent it from having a flattened side from lying in the bottom of the jar (the brain is heavier than water and therefore sinks).
The heart is weighed and opened along the pathway of normal blood flow using the bread knife or scissors. Old-time pathologists look down on prosectors who open the heart with scissors, rather than the bread knife, because, while the latter takes more skill and care, it is much faster and gives more attractive cut edges than when scissors are used. The coronary arteries are examined by making numerous crosscuts with a scalpel.
The larynx and trachea are opened longitudinally from the rear and the interior examined. The thyroid gland is dissected away from the trachea with scissors, weighed, and examined in thin slices. Sometimes the parathyroid glands are easy to find, other times impossible.
The bloc containing the abdominal organs is turned over so that the back side is up. The adrenal glands are located in the fatty tissue over the kidneys (they are sometimes difficult to find) and are removed, weighed, sliced, and examined by the prosector.
The liver is removed with scissors from the rest of the abdominal organs, weighed, sliced with a bread knife, and examined. The spleen is similarly treated.
The intestines are stripped from the mesentery using scissors (the wimpy method) or bread knife (macho method). The intestines are then opened over a sink under running water, so that all the feces and undigested food flow out. As one might imagine, this step is extremely malodorous. The resultant material in the sink smells like a pleasant combination of feces and vomitus. The internal (mucosal) surface of the bowel is washed off with water and examined. It is generally the diener's job to "run the gut," but usually a crusty, senior diener can intimidate a young first- year resident prosector into doing this ever-hated chore. Basically, whichever individual has the least effective steely glare of disdain is stuck with running the gut.
The stomach is then opened along its greater curvature. If the prosector is lucky, the patient will have not eaten solid food in a while. If not, the appearance of the contents of the stomach will assure the prosector that he will not be eating any stews or soups for a long time. In either case, the smell of gastric acid is unforgettable.
The pancreas is removed from the duodenum, weighed, sliced and examined. The duodenum is opened longitudinally, washed out, and examined internally. The esophagus is similarly treated.
The kidneys are removed, weighed, cut lengthwise in half, and examined. The urinary bladder is opened and examined internally. In the female patient, the ovaries are removed, cut in half, and examined. The uterus is opened along either side (bivalved) and examined. In the male, the testes are typically not removed if they are not enlarged. If it is necessary to remove them, they can be pulled up into the abdomen by traction on the spermatic cord, cut off, cut in half, and examined.
The aorta and its major abdominal/pelvic branches (the renal, celiac, mesenteric, and iliac arteries) are opened longitudinally and examined.
Most of the organs mentioned above are sampled for microscopic examination. Sections of the organs are cut with a bread knife or scalpel and placed in labeled plastic cassettes. Each section is the size of a postage stamp or smaller and optimally about three millimeters in thickness. The cassettes are placed in a small jar of formalin for fixation. They are then "processed" in a machine that overnight removes all the water from the specimens and replaces it with paraffin wax. Permanent microscopic sections (five microns, or one two-hundredth of a millimeter thick) can be cut from these paraffin sections, mounted on glass slides, stained, coverslipped, and examined microscopically. The permanent slides are usually kept indefinitely, but must be kept for twenty years minimum.
Additional small slices of the major organs are kept in a "save jar," typically a one-quart or one-pint jar filled with formalin. Labs keep the save jar for a variable length of time, but at least until the case is "signed out" (i.e., the final written report is prepared). Some labs keep the save jar for years. All tissues that are disposed of are done so by incineration.
A note on dissection technique: All of the above procedures are done with only four simple instruments -- a scalpel, the bread knife, scissors, and forceps (which most medical people call "pick-ups." Only scriptwriters say "forceps"). The more handy the prosector, the more he relies on the bread knife, sometimes making amazingly delicate cuts with this long, unwieldy-looking blade. The best prosectors are able to make every cut with one long slicing action. To saw back and forth with the blade leaves irregularities on the cut surface which are often distracting on specimen photographs. So the idea is to use an extremely sharp, long blade that can get through a 2000-gram liver in one graceful slice. Some old-time purist pathologists actually maintain their own bread knives themselves and let no one else use them. Such an individual typically carries it around in his briefcase in a leather sheath. This would make an excellent fiction device, which, to my knowledge, has not been used. Imagine a milquetoast pathologist defending himself from a late-night attacker in the lab, with one desperate but skillful slash of the bread knife almost cutting the assailant in half!
Note on the appearance of the autopsy suite: Toward the end of the autopsy procedure, the room is not a pretty sight. Prosectors vary markedly in how neat they keep the dissection area while doing the procedure. It is legendary that old-time pathologists were so neat that they'd perform the entire procedure in a tux (no apron) right before an evening at the opera (pathologists are noted for their love of classical music and fine art). Modern prosectors are not this neat. Usually, the autopsy table around the patient is covered with blood, and it is very difficult not to get some blood on the floor. We try to keep blood on the floor to a minimum, because this is a slippery substance that can lead to falls. The hanging meat scales used to weigh the organs are usually covered with or dripping with blood. The chalk that is used to write organ weights on the chalkboard is also smeared with blood, as may be the chalkboard itself. This is an especially unappetizing juxtaposition.
The empty trunk looks like the hull of a ship under construction, the prominent ribs resembling the corresponding structural members of the ship. In many institutions, the sliced organs are just poured back into the open body cavity. In other places, the organs are not replaced but just incinerated at the facility. In either case, the chest plate is placed back in the chest, and the body wall is sewn back up with baseball stitches, so that the final wound again resembles a "Y."
The diener rinses the body off with a hose and sponge, covers it with a sheet, and calls the funeral home for pick- up. As one might imagine, if the organs had not been put back in the body, the whole trunk appears collapsed, especially the chest (since the chest plate was not firmly reattached to the ribs). The mortician must then remedy this by placing filler in the body cavity to re-expand the body to roughly normal contours.
Ultimately, what is buried/cremated is either 1) the body without a brain and without any chest, abdominal, or pelvic organs, or 2) the body without a brain but with a hodgepodge of other organ parts in the body cavity.
Assuming good fixation of an adult brain, it is removed from the formalin and rinsed in a running tap water bath for several hours to try to cut down on the discomforting, eye- irritating, possibly carcinogenic formalin vapors. The cerebrum is severed from the rest of the brain (brainstem and cerebellum) by the prosector with a scalpel. The cerebellum is severed from the brainstem, and each is sliced and laid out on a tray for examination. The cerebrum is sliced perpendicularly to its long axis and laid out to be examined. Sections for microscopic processing are taken, as from the other organs, and a few slices are held in "save jars." The remainder of the brain slices is incinerated.
Copyright (c) 1994, 1995, Edward O. Uthman. This material may be reformatted and/or freely distributed via online services or other media, as long as it is not substantively altered. Authors, educators, and others are welcome to use any ideas presented herein, but I would ask for acknowledgment in any published work derived therefrom.